TABLE 3.
Summary of the proteins identified from 2DE gels of B. cereus DL5 whole-cell protein extracts
| Protein spot | Protein identity | Function | N-terminal sequence | Estimated from gel
|
Calculated from sequence
|
Accession no.
|
|||
|---|---|---|---|---|---|---|---|---|---|
| Size (kDa) | pI | Size (kDa) | pI | B. cereus databasea | SWISS-PROT protein database | ||||
| B5a | Pyruvate dehydrogenase E1 component, beta subunit (PdbB) | Pyruvate + lipoamide → S-acetyl-dihydro-lipoamide + CO2 | QMTMIQAITD | 36 | 4.90 | 35.23 | 4.75 | RZC01921 | P21882 |
| B11b | Unknown | MNVKRHM | 82 | 4.80 | |||||
| D5h | l-Lactate dehydrogenase (LctE) | Pyruvate + NADH → l-lactate + NAD+ | MKKGINRVVL | 34 | 5.50 | 34.79 | 4.99 | RZC05932 | P13714 |
| E1d | Hypothetical cytosolic protein | Unknown | AKDFYSAIEDRRS | 24 | 5.65 | 23.07 | 5.21 | RZC03248 | A83323 |
| E7g | cOTCase | l-Citrulline + Pi → carbamoyl phosphate + ornithine | MLMTRPNLKGRS | 39 | 5.70 | 37.46 | 5.35 | RZC04697 | O86132 |
| E7h | cOTCase | l-Citrulline + Pi → carbamoyl phosphate + ornithine | MLMTRPNLKGRS | 39 | 5.80 | 37.46 | 5.35 | RZC04697 | O86132 |
| E9p | Unknown | MPPVVI | 54 | 6.10 | |||||
| G1b | YhbH light-repressed protein A | Unknown | MKFNI | 23 | 6.20 | 21.08 | 5.63 | RZC04452 | P28368 |
a
Accession number obtained from the B. cereus database website (http://wit.Integratedgenomics.com/Public/IG_Release.html).
b
Accession number obtained from the SWISS-PROT protein database website (http://www.expasy.ch/sprot/).