TABLE 3.
Michaelis-Menten kinetic parameters for the degradation of toluene, ethylbenzene, and xylenes by toluene-grown cells of Cladophialophora sp. strain T1, as measured in batch incubation preparations (120 rpm, 25°C)
| Substrate | Vmax (μmol g−1 h−1)a |
Kmb
|
Vmax/Km (liters g−1 h−1) | |
|---|---|---|---|---|
| Water (μM) | Air equivalent (mg m−3) | |||
| Toluene | 75 ± 5c | 2.7 ± 1.0c | 66 | 30.7 |
| Ethylbenzene | 45 | 5.8 | 195 | 7.8 |
| o-Xylene | 21 | 4.2 | 88 | 4.4 |
| m-Xylene | 19 | 3.8 | 112 | 5.0 |
| p-Xylene | 2 | 1.1 | 33 | 1.8 |
a
Measured rates (n ≥ 6, r2 ≥ 0.99).
b
Data from Lineweaver-Burk plot linearization (n ≥ 8, r2 ≥ 0.97).
c
Average ± standard deviation based on three independent experiments.