TABLE 2.
Growth parameters of M. austroafricanum IFP 2012 on MTBE and its metabolites
Growth substrate | Maximum growth rate (h−1)a,b | Biomass yield (g/g)b | Carbon recovery (%)b,c |
---|---|---|---|
MTBE | NDd | 0.436 (0.01) | 99.7 (3.9) |
TBA | 0.039 (0.001) | 0.609 (0.02) | 97.3 (1.9) |
HIBA | 0.061 (0.001) | 0.422 (0.01) | 101.6 (1.8) |
Substrates were added at final concentrations of 350 mg/liter for TBA and 250 mg/liter for HIBA.
Mean (standard deviation) for three samples.
Substrates were added at final concentrations of 625 mg/liter for TBA and HIBA and 200 mg/liter−1 for MTBE. The initial OD600 were 0.1 for the experiments with TBA and HIBA and 0.4 for the experiment with MTBE. At the end of growth, the amount of biomass, the amount of CO2 produced, and the residual carbon source amount were determined.
ND, not determined.