TABLE 3.
Detection of N. fowleri in natural waters by RPE-Cy5 labeling associated with solid-phase cytometry (ChemScan) and plate counts (MPN)a
| Samples date (day/mo./yr)b | ChemScan resultc | No. of validated cellsd | No. of N. fowleri amoebae/liter
|
|
|---|---|---|---|---|
| ChemScan | MPN | |||
| 07/05/01 | 141.20 ± 52.70 | 13.40 ± 3.51 | 7,504 ± 1,964 | 5,423 ± 2,336 |
| 12/05/01 | 68.40 ± 51.55 | 14.40 ± 4.39 | 16,128 ± 4,920 | 16,095 ± 6,324 |
| 15/05/01 | 33.80 ± 11.67 | 12.80 ± 4.02 | 6,048 ± 2,183 | 5,423 ± 2,336 |
| 17/05/01 | 426.60 ± 44.25 | 8.00 ± 1.58 | 4,480 ± 885 | 3,477 ± 1,337 |
| 18/05/01 | 321.60 ± 153.35 | 4.00 ± 2.35 | 2,240 ± 1,313 | 1,959 ± 689 |
a
Values are means ± standard deviations of five subsamples.
b
Water samples were collected from Nogent nuclear power plant site.
c
Counts determined by the ChemScan after discrimination.
d
Counts obtained after microscopic validation.