REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-CPNE5	Proteintech	Cat# 18097-1-AP; RRID:AB_2878499
anti-FAS	Abcam	Cat# ab271016; RRID: AB_3712731
anti-ANP	ABclonal	Cat# A14755; RRID: AB_2761631
anti-BNP	ABclonal	Cat# A23996; RRID: AB_3712732
anti-β-actin	ABclonal	Cat# AC038; RRID: AB_2863784
anti-Flag	Proteintech	Cat# 66008-4-Ig; RRID: AB_2918475
anti-Cleaved Caspase3	Cell Signaling Technology	Cat# 9661; RRID: AB_2341188
anti-Cleaved Caspase8	Cell Signaling Technology	Cat# 8592; RRID: AB_10891784
anti-FADD	Proteintech	Cat# 14906-1-AP; RRID: AB_2100486
anti-SEC13	Proteintech	Cat# 15397-1-AP; RRID: AB_2186234
anti-LC3	Proteintech	Cat# 14600-1-AP; RRID: AB_2137737
anti-Puromycin	KeraFASt	Cat# EQ0001; RRID: AB_2620162
anti-p-eiF2α	Cell Signaling Technology	Cat# 3398; RRID: AB_2096481
anti-SQSTM1/P62	Cell Signaling Technology	Cat# 5114; RRID: AB_10624872
anti-CALCOCO1	Proteintech	Cat# 19843-1-AP; RRID: AB_10637265
anti-cardiac troponin T	Proteintech	Cat# 68300-1-Ig; RRID: AB_3085051
anti-CPNE5	Novus	Cat# NBP1-84406; RRID: AB_11013078
anti-ERp72/ER marker	Proteintech	Cat# 14712-1-AP; RRID: AB_2160973
anti-LAMP1/Lysosome marker	Santa Cruz	Cat# sc-20011; RRID: AB_626853
Bacterial and virus strains
AAV9-OE-Cpne5	Hanheng Biotechnology Co., Ltd. (Shanghai, China)	N/A
AAV9-OE-Fas	Hanheng Biotechnology Co., Ltd. (Shanghai, China)	N/A
Biological samples
heart tissue (mus)	This paper	N/A
Chemicals, peptides, and recombinant proteins
collagenase II	Worthington	LS004176
Opti-MEM™ Medium	Thermo Fisher Scientific	31985070
Lipofectamine™3000	Thermo Fisher Scientific	L3000-015
CHX	MCE	HY-12320
Chloroquine	MCE	HY-17589A
MG132	Selleck Chemicals	S2619
FASL protein	MCE	HY-P74157
puromycin 2HCl	Selleck Chemicals	S7417
phosphatase inhibitors	Epizyme	GRF101
protease inhibitors	Epizyme	GRF102
IP lysis buffer	Thermo Fisher Scientific	87787
Protein A/G magnetic beads	MCE	HY-K0202
Trizol Reagent	Thermo Fisher Scientific	15596026
Prime Script™ RT Master Mix Kit	Takara	RR036A
Experimental models: Organisms/strains
Mouse: Male CPNE5 knockout (KO) mice	Cyagen Biosciences Biotechnology Co., Ltd.(Suzhou, China)	N/A
Oligonucleotides
mouse CPNE5 siRNA sequence: Sense 5’-GCACCGAGGUCAUAGAUAATT-3’; Antisense 5’-UGUUUAGAU AGAUCCGGGCTT-3’.	Genepharma Biotechnology Co., Ltd. (Jiangsu, China)	N/A
mouse FAS siRNA sequence: Sense 5’-AAGAAGAAGUUCACAGAAATT-3’; Antisense 5’-UUUCUGUGAA CUUCUUCUUTT-3’.	Genepharma Biotechnology Co., Ltd. (Jiangsu, China)	N/A
mouse CPNE5 forward primer 5’-ATGTTGACTCCAAGAGCCCG-3’ mouse CPNE5 reverse primer 5’-CATCCAAAGACCACTTCCGTT-3’	This paper	N/A
mouse FAS forward primer 5’-GCTTGCTGGCTCACAGTTAAG-3’ mouse FAS reverse primer 5’-GCTCAGCCTAGTTTTCAGGTT-3’	This paper	N/A
mouse β-actin forward primer 5’-TAGGCACCAGGGTGTGATGG-3’ mouse β-actin reverse primer 5’-CATGGCTGGGGTGTTGAAGG-3’	This paper	N/A
Software and algorithms
ImageJ software (version 4.0)	National Institutes of Health	https://imagej.net/ij/.
GraphPad Software	Graphpad Software Inc.	https://www.graphpad.com/scientific-software/prism/