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Determination of the amount of anatoxin-a(s) by progressive inhibition. In this experiment, 10, 7, 5, 3, 2, 1, and 0.5 pmol of AChE were mixed with aliquots of an anatoxin-a(s)-containing sample. Inhibition was monitored over time. The plateaus correspond to the free enzyme remaining when all of the neurotoxin had reacted with the AChE.
