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. 2005 Oct 3;102(41):14671–14676. doi: 10.1073/pnas.0502480102

Fig. 7.

Fig. 7.

fng 3′ enhancer RNAi luciferase assay. Mirr mediates transcriptional repression on the fng enhancer. Blue indicates luciferase activity in S2 cells transfected with a pGL3 reporter containing the fng-IBS, treated with dsRNA to GFP. Magenta indicates luciferase units from S2 cells transfected with the same construct but treated with dsRNA to mirr. Yellow indicates luciferase activity from S2 cells with a point mutation in the fng-IBS, treated with RNAi to GFP. Light blue indicates luciferase activity from S2 cells containing the mutated enhancer, treated with RNAi to mirr. (Inset) Western blot from S2 cells treated with dsRNA to either GFP (Left) or Mirr (Right) shows dramatic reduction of Mirr protein levels specifically in response to Mirr RNAi.