Table 2.

Primers and probes used in the experiments.

Gene	Forward primer	Reverse primer	Application
circKCNK2	CCGTTAGGAAACACCTCCAA	GGCAGATTTAGGATCCAGCA	qRT-PCR
circFANCA	AGCTGGACTGCGAGAGAGAG	GTGGAAGAACTGCTCGCATC	qRT-PCR
circPLCL2	CCATCCCAGTCCAGTTCCTA	AAGGGCCCTAGCTCAAGAAG	qRT-PCR
KCNK2	CTCTTGGCTGGAGTTGGAGA	GCCACAAAGAGTACACAGCC	qRT-PCR
FANCA	GCTGCTTATCTCCAGGCCTT	GTGTGTCCAGAGAGAGAGGG	qRT-PCR
PLCL2	GGTTGCCGGAGTGTTGAATT	TGTGCTGAACCATTACCTTCTG	qRT-PCR
IL-11	ATGAACTGTGTTTGCCGCC	CCGTCAGCTGGGAATTTGTC	qRT-PCR
IL-8	CAGTTTTGCCAAGGAGTGCT	ACTTCTCCACAACCCTCTGC	qRT-PCR
IL-6	GACAGCCAACTACGATGATG	GCAAGTCTCCTCATTGAATCC	qRT-PCR
IL-1	TGGAAAAGCGATTTGTCTTCAA	CAGTTATATCCTGGCCGCCT	qRT-PCR
IL-10	GCCAAGCCTTGTCTGAGATG	TGAGGGTCTTCAGGTTCTCC	qRT-PCR
TNF-alpha	GTCAACCTCCTCTCTGCCAT	CCAAAGTAGACCTGCCCAGA	qRT-PCR
RUNX2	CTGTGGTTACTGTCATGGCG	AGGTAGCTACTTGGGGAGGA	qRT-PCR
PTHrP	TTACGGCGACGATTCTTCC	CAGTCACTCCAGAGTCTAACC	qRT-PCR
CXCL-6	CTGGTCCTGTCTCTGCTGTG	CACCTTGGAGCACTGCGG	qRT-PCR
RANKL	TGGGCCAAGATCTCCAACAT	GGTGCTTCCTCCTTTCATCAG	qRT-PCR
VEGFA	GACGGACAGACAGACAGACA	CGAGAACAGCCCAGAAGTTG	qRT-PCR
ET-1	GAGAAACCCACTCCCAGTCC	CCAGGTGGCAGAAGTAGACA	qRT-PCR
COX-2	TGACCCAGAACTACTTTTCAACA	TCCATGTTCCAGCAATATAGAGT	qRT-PCR
RSPO-2	GCGAATGGGGAACTTGTAGC	TGCCTCATTGTCATCTTGCA	qRT-PCR
MCP-1	CAATCAATGCCCCAGTCACC	GGGACACTTGCTGCTGGT	qRT-PCR
DKK-1	AGGTTCTGTTTGTCTCCGGT	CTCCACAGTAACAACGCTGG	qRT-PCR
IGF-1	CCACAGGGTATGGCTCCAG	CTTCTGGGTCTTGGGCATGT	qRT-PCR
TGF-beta	ATCGACATGGAGCTGGTGAA	CTCCTTGGCGTAGTAGTCGG	qRT-PCR
M-CSF	ACCAAGCCTGATTGCAACTG	CTGCCTGGATCCACTGTGT	qRT-PCR
IFN-alpha	GTCCTCCATGAGCTGATCCA	GTTTCTCCCACCCTCTCCTC	qRT-PCR
EDC4	AGCATCTGCGGGACATACTC	CTCCAGAGAGGCAAATGACC	qRT-PCR
hnRNPU	CTGTTCCCGCATGTTCTCTG	CCCTGGATTTTCTGCTGCAT	qRT-PCR
β-Actin	GGACTTCGAGCAAGAGATGG	AGGAAGGAAGGCTGGAAGAG	qRT-PCR
U1	TGAAGGCGCTTTTCTCATGG	CAGGGGAAAGCACGAACG	qRT-PCR
GAPDH	CATGAGAAGTATGACAACAGCC	AGTCCTTCCACGATACCAAAGT	qRT-PCR
Probe-circKCNK2	Biotin 5’- AGGTGCCGCCACTATGGTTG		ISH/FISH