TABLE 1.
Specificity of real-time PCR for diatom and pelagophyte DNA
| Clone | Phylogenetic association | GenBank accession no. | Amplification | Detection by real-time PCR |
|---|---|---|---|---|
| Positive controls | ||||
| + | + | |||
| P994AH8 | Diatom | AF381735 | + | + |
| P994CH1 | Pelagophyte | AF381685 | + | + |
| P994CH2 | Diatom | AF381686 | + | + |
| P994CH3 | Diatom | AF381677 | + | + |
| P994CH22 | Diatom | AF381676 | + | + |
| P994FH13 | Diatom | AF381681 | + | + |
| P994HH14 | Diatom | AF381684 | + | + |
| P994HH7 | Pelagophyte | AF381691 | + | + |
| P994AH8 | Diatom | AF381735 | + | + |
| Negative controls | ||||
| P994AH1 | Prymnesiophyte | AF381673 | + | − |
| P994AH5 | Prymnesiophyte | AF381733 | + | − |
| P994AH7 | Prymnesiophyte | AF381734 | + | − |
| P994AH12 | Prymnesiophyte | AF381673 | + | − |
| P994AH28 | Prymnesiophyte | AF381732 | + | − |
| P994CH9 | Prymnesiophyte | AF381741 | + | − |
| P994CH25 | Eustigmatophyte | AF381740 | + | − |
| P994HH1 | Prymnesiophyte | AF381752 | + | − |
| P994FY9 | Prochlorococcus | AF381660 | − | − |