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Determination of L. lactis pHi as a function of pHex by use of ratiometric GFP and cFDAse. The pHi of cells transformed with ratiometric GFP (closed circles) was obtained by measuring the R410/430 and converting the obtained value to the correlating pHi. For validation, pHi was also obtained by staining the cells with cFDAse (open circles) and measuring the R490/435. Each point represents the mean value for 20 individual cells, with error bars indicating the standard deviations.
