TABLE 1.
Identification of and AHL production by 43 bacterial strains isolated from detrital and algal aggregates, based on partial (800-bp) 16S rRNA gene sequencing
| Phylum or group | Strain | Identification by GenBank alignment | % Homology to GenBank sequence | AHLb |
|---|---|---|---|---|
| α-Proteobacteria | HP39 | AF025321, α-Proteobacterium KAT8 | 99 | − |
| HP40 | AF025321, α-Proteobacterium KAT8 | 98 | − | |
| HP24 | U70680, Rhodobacter-Roseobacter strain OM42 | 94 | − | |
| HP12 | AF359535, Roseobacter strain ATAM407 | 98 | + | |
| HP30 | AJ294355, Roseobacter strain 667-19 (uncultured) | 98 | + | |
| HP32 | AF359535, Roseobacter strain ATAM407 | 97 | + | |
| HP44w | Y15343, Roseobacter strain PRLISTO6 | 98 | − | |
| HP27 | X91814, Sphingobacterium comitans | 92 | − | |
| HP18 | AY007681, Sphingomonas sp. (unknown) | 98 | − | |
| HP22 | AJ318163, uncultured α-Proteobacterium Blri | 94 | − | |
| HP28 | AJ290003, uncultured α-Proteobacterium FukaNS | 100 | − | |
| HP33 | AF345550Rhizobium sp. strain SDW052 | 99 | − | |
| AF388033, A. tumefaciens | 99 | |||
| HP13 | AF359546, marine bacterium SCRIPPS 739 | 96 | − | |
| CFBa | HP34 | AF367847, bacterium KM1 | 96 | − |
| HP25 | AF277514, Cellulophaga strain SIC.834 (uncultured) | 98 | − | |
| HP2 | AF235114, Cytophaga strain KTO2ds22 | 98 | − | |
| HP14 | AF235114, Cytophaga strain KTO2ds22 | 98 | − | |
| HP19 | AF235114, Cytophaga strain KTO2ds22 | 98 | − | |
| HP31 | AF235114, Cytophaga strain KTO2ds22 | 97 | − | |
| HP35 | AF235114, Cytophaga strain KTO2ds22 | 98 | − | |
| HP20 | AF321022, Frigobacterium sp. strain GOB | 98 | − | |
| HP11 | M58792, Microscilla furvescens | 90 | − | |
| HP23 | AF287047, uncultured strain EC-B39 | 92 | − | |
| γ-Proteobacteria | HP3 | AF062642, Alcanivorax borkumensis | 98 | − |
| HP26 | L10938, Alteromonas macleodii | 98 | − | |
| HP1 | AJ002006, Curacaobacter baltica | 98 | − | |
| HP9 | AJ002006, C. baltica | 97 | − | |
| HP41 | AJ302707, Marinobacter strain ME108 | 97 | − | |
| HP6 | AJ000647, Marinobacter strain PCOB-2 | 99 | − | |
| HP15 | AJ000647, Marinobacter strain PCOB-2 | 98 | − | |
| HP36 | AJ000647, Marinobacter strain PCOB-2 | 98 | + | |
| HP4 | AJ295154, Oleiphilus messinensis | 93 | − | |
| Bacillus-Clostridium | HP16 | AJ296095, Bacillus sp. strain OS-5 | 96 | − |
| HP17 | AJ296095, Bacillus sp. strain OS-5 | 98 | − | |
| HP29 | AJ296095, Bacillus sp. strain OS-5 | 98 | − | |
| HP38 | AJ296095, Bacillus sp. strain OS-5 | 98 | ||
| HP21 | AY030327, Bacillus pumilis KL-052 | 99 | − | |
| HP8 | AY038905, marine bacterium SE165 | 97 | − | |
| HP10 | AF275714, Haeler soda lake bacterium Z6 | 99 | − | |
| Actinomycete | HP42 | AJ344143, Citrococcus muralis 1b | 98 | − |
| HP5 | AF321022, Frigobacterium sp. strain GOB | 98 | − | |
| HP7 | AF197036, Arthrobacter sp. strain SMCC G980 | 97 | − | |
| Cyanobacterium | HP43 | D90916, Synechocystis sp. strain PCC6803 | 100 |
a
CFB, Cytophaga-Flavobacterium-Bacteroides.
b
Presence (+) or absence (−) of AHL as tested by using the A. tumefaciens monitor system. A positive reaction was not reproducible for strain HP43.