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. 2002 Aug;68(8):3956–3964. doi: 10.1128/AEM.68.8.3956-3964.2002

FIG. 3.

FIG. 3.

Southern analysis of transformants ALKO3530 and ALKO3574 in the which cbh1 locus has been replaced with the 9.2-kb NotI fragment from pALK537. (A) Genomic DNA was digested with XhoI. Hybridization was done with a cbh1 probe. Lane 1, VTT-D-79125; lane 2, ALKO3530; lane 3, ALKO3574; lane 4, molecular weight marker λHindIII. (B) Genomic DNA was digested with XhoI. Hybridization was performed with the 9.2-kb NotI fragment used for the transformations. Lanes 1 and 2, molecular weight markers λHindIII and λEcoRI-HindIII; lane 3, ALKO3530; lane 4, ALKO3574; lane 5, VTT-D-79125. (C) Schematic presentation of the organization of the cbh1 chromosomal locus in the host strain and the transformants, showing the XhoI cleavage site.