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Southern analysis of the transformant ALKO3529 and the host strain VTT-D-79125. (A) Genomic DNA was digested with XhoI. Hybridization was done with a cbh1 probe. Lane 1, VTT-D-79125; lane 2, ALKO3529; lanes 3 and 4, molecular weight markers λHindIII and λEcoRI-HindIII. (B) Genomic DNA was digested with PvuI. Hybridization was done with an egl2 probe. Lane 1, molecular weight marker λHindIII; lane 2, ALKO3529; lane 3, molecular weight marker λHindIII; lane 4, VTT-D-79125.
