TABLE 2.
Production of cellulases by the host strain VTT-D-79125, transformants ALKO3529, ALKO3574, and ALKO3530, and T. reesei EGI-overproducing strains ALKO2697, ALKO2698, and ALKO2656a
| Strain | cbh1 Southern analysis result | egl2 cassette copy no. | Secreted protein (mg/ml) | Endoglucanase (HEC) (nkat/ml) | β-glucanase (nkat/ml) | FPU/ml | β-glucosidase (nkat/ml) | CBHI (mg/ml) | CBHII (mg/ml) | EGI (mg/ml) |
|---|---|---|---|---|---|---|---|---|---|---|
| VTT-D-79125 | + | 0 | 9.2 | 1,200 ± 0 | 9,300 ± 500 | 5.3 ± 0.1 | 211 ± 10 | 3.7 | 0.051 | 0.361 |
| ALKO3529 | + | 2 | 10.1 | 3,400 ± 100 | 23,000 ± 2,000 | 6.0 ± 0.7 | 239 ± 5 | 3.4 | 0.069 | 0.587 |
| ALKO3530 | − | 2 | 8.7 | 3,600 ± 100 | 25,000 ± 1,000 | 2.1 ± 0.2 | 320 ± 5 | NAb | 0.085 | 0.590 |
| ALKO3574 | − | 1 | 7.4 | 2,800 ± 50 | 19,400 ± 1,000 | 1.7 ± 0.2 | 281 ± 12 | NA | NA | 0.650 |
| ALKO2697 | − | 0 | 9.2 | 2,600 ± 100 | 20,200 ± 500 | 1.8 ± 0.1 | NA | NA | NA | NA |
| ALKO2698 | − | 0 | 7.6 | 2,300 ± 50 | 15,700 ± 500 | 1.6 ± 0 | 200 ± 5 | NA | NA | NA |
| ALKO2656 | − | 0 | 9.1 | 2,700 ± 500 | 20,200 ± 3,000 | 1.4 ± 0.4 | 231 ± 5 | NA | NA | NA |
a
Strains were grown for 7 days in cellulase-inducing medium; the results are the average from three flasks. The standard errors are shown.
b
NA, not analyzed.