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Production of cellulases by the host strains VTT-D-79125 and ALKO2698 and by the transformant ALKO3528
| Strain | Secreted protein (mg/ml) | Endoglucanase (HEC) (nkat/ml) | β-glucanase (nkat/ml) | FPU/ml | Southern analysis
|
|
|---|---|---|---|---|---|---|
| cbh1 | cbh2 | |||||
| VTT-D-79125 | 9.0 | 1,300 ± 50 | 11,500 ± 1,000 | 4.8 ± 0.8 | + | + |
| ALKO2698 | 7.9 | 2,600 ± 100 | 15,300 ± 1,000 | 1.4 ± 0.1 | − | + |
| ALKO3528 | 8.5 | 5,100 ± 400 | 27,800 ± 2,000 | 0.2 ± 0.1 | − | − |
