TABLE 2.
Stability against and activity in water-miscible organic solvents
| Organic solvent | Residual activity after 1 h incubationa (%)
|
Activity in 50% solventb (U/mg) | |
|---|---|---|---|
| 50% concn | 80% concn | ||
| None | 100 | 100 | 4,053 ± 241 |
| Methanol | 109 ± 2 | 89 ± 4 | 556 ± 9 |
| Ethanol | 105 ± 5 | 103 ± 1 | 101 ± 41 |
| 2-Propanol | 110 ± 2 | 94 ± 1 | 42 ± 14 |
| Acetonitrile | 117 ± 9 | 116 ± 5 | 119 ± 53 |
| Dimethyl sulfoxide | 101 ± 2 | 90 ± 2 | 2,148 ± 131 |
| Dimethylformamide | 124 ± 3 | 96 ± 1 | 296 ± 28 |
a
Incubation was carried out at 30°C, and activity measurements were at 70°C.
b
Activity measurements were carried out at 70°C. Extinction coefficients for each solvent (50%) are indicated in Materials and Methods.