Figure 1.

The PMK-1 pathway regulated by stress response. (A,B) Activation of PMK-1 by oxidative stress. Animals were treated with oxidative stress agents. Extracts prepared from each animal were immunoblotted (IB) with anti-phospho p38 and anti-PMK-1. (t-BOOH) t-butyl peroxide. (C,D) Stress sensitivity in mutants. (C) Animals were scored for survival following exposure to sodium arsenite (5 mM) in M9 buffer for the indicated times. (D) Wild-type (N2) and sek-1 mutant animals harboring control vector or transgene as an extrachromosomal array were scored for survival after they had been placed in M9 containing sodium arsenite (5 mM) for 24 h.