TABLE 2.
Virus | Slopeb | Correlation (r) | Adsorption groupc |
---|---|---|---|
MS2 | −170 ± 26 A | 0.88 | I |
E1 | −160 ± 17 A | 0.87 | I |
φX174 | −130 ± 26 AB | 0.71 | I |
CB4 | −115 ± 22 B | 0.82 | |
T3 | −110 ± 21 B | 0.81 | |
T7 | −110 ± 21 B | 0.81 | |
CB5 | −105 ± 15 B | 0.89 | |
E4 | −100 ± 19 B | 0.82 | |
f2 | −95 ± 15 BC | 0.87 | |
P1 | −65 ± 20 C | 0.80 | II |
T4 | −65 ± 10 C | 0.88 | II |
T2 | −30 ± 4 D | 0.91 | II |
E5 | −6 ± 8 E | 0.08 |
The amount of virus adsorbed as the ionic strength of the solution was increased was determined and plotted as a percentage of the number of viruses in the portion of the solution that was not mixed with DEAE-Sepharose.
The slope was determined by dividing the percentage of virus adsorbed by the ionic strength of the solution. The values are means ± standard deviations for triplicate determinations. Values followed by the same letter are not significantly different at a P value of >0.05. The weakest electrostatic interaction is represented by the slope of −170 ± 26 (MS2), and the strongest electrostatic interaction is represented by the slope of −6 ± 8 (E5).
Virus groups described by Gerba and Goyal (10).