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. 2005 Oct 4;2(10):e264. doi: 10.1371/journal.pmed.0020264

Figure 5. ADMA Alters BMP2K Gene Expression and Protein Levels.

Figure 5

(A) BMP2K is increased more than 2-fold in HCAEC following 24-h ADMA treatment (2 μM and 100 μM ADMA increased expression by 2.304-fold [p = 0.00128] and 2.695-fold [p < 0.001] respectively).

(B) BMP2K protein levels are increased in HCAEC following 24-h treatment with ADMA (2 and 100 μM) as determined by Western blotting, using a commercially available BMP2K antibody (Orbigen). Densitometry of the BMP2K band was carried for each of the blots from four separate experiments (untreated versus 2 μM: p < 0.05; and untreated versus 100 μM: p < 0.01, n = 4). The inset blot is representative of four separate experiments, where equal amounts of protein were loaded in each lane.