Figure 3. Canine Cellular Immune Response to Vaccination with Recombinant Ac-APR-1.

Cell proliferation of whole blood cells from vaccinated (APR-1) and control dogs (AS03) when stimulated with concanavalin A (A) or recombinant Ac-APR-1 (B) before (day 0) and after the final immunization (day 51). The p-value comparing the mean differences between the vaccinated group and controls is denoted. Detection of secreted IFN-γ in whole blood cultures taken from vaccinated and control dogs before and after immunization (C). Mean cytokine concentrations are indicated in pg·ml⁻¹ with standard error bars. Statistically significant differences are indicated above the bars by p-values. APR, stimulated with recombinant APR-1; NS, non-stimulated cultures.