Skip to main content
. 2025 Aug 18;12:1647236. doi: 10.3389/fvets.2025.1647236

Table 2.

Factors significantly associated with saliva cortisol concentrations the night prior to transportation (T-1) and after transport (T1).

Outcome variable Explanatory factor(s) Significance
P=
Effect size
T-1 Cortisol concentration
Travel history 0.029 Nil/unknown 12.8 (8.2–17.4), occ/freq 7.9 (5.8–9.9) nmol/L**
T1 Cortisol concentration
Waypoint temperature <0.001 rs = 0.572
Arrival temperature 0.002 rs = 0.535
Waypoint humidity <0.001 rs = −0.585
Arrival humidity 0.001 rs = −0.569
Distance traveled 0.002 rs = −0.533
Duration of travel 0.004 rs = −0.514
Time of arrival 0.011 rs = −0.457
Time of sampling 0.018 rs = −0.428
Interval btw arrival & sampling 0.002 rs = 0.535
Trip <0.001 Trip 8 16.2 (13.0–19.0), other trips 5.8 (4.5–9.5) nmol/L*
Sweat score <0.001 rs = 0.653
Abnormal demeanour 0.005 rs = 0.497

*Median (IQR); **Mean (95% CI). occ, occasional; freq, frequent; btw, between; IQR, interquartile range (non-parametric data); 95% CI, 95% confidence interval (parametric data). Interactions between continuous explanatory data with continuous outcomes data were explored by Pearson (parametric) or Spearman (non-parametric) correlation; effects of categorical explanatory factors on continuous data were explored by analysis of variance or t-test (or non-parametric equivalent, as appropriate), and effects of categorical explanatory data on categorical outcomes were evaluated using Spearman correlation. No significant interactions were observed for salivary cortisol concentrations immediately prior to departure (T0).