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PCR analysis of environmental surface water samples for Shigella-specific virulence genes. Products of a PCR assay for the ipaH gene (A) and a multiplex PCR assay for the ipaBCD and stx1 genes (B) are shown. Lanes 1 through 7 contain PCR products of environmental water samples, and lane 8 contains a positive control strain. Lane 9 shows the 1-kb DNA ladder (Bethesda Research Laboratories) used as molecular size markers.
