TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and/or relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| Pseudomonas | Prototroph | 12 |
| B10 | ||
| B10CA1 | psbA::cat site-specific insertion mutant | 2 |
| P. aeruginosa | ||
| PAO1 (ATCC 15692) | Prototroph | American Type Culture Collection |
| PALS124 | pvdA | 26 |
| PALS106 | pvdC1 | 26 |
| PALS125 | pvdC3 | 26 |
| Plasmids | ||
| pMP220 | Broad-host-range, low-copy-number promoter-probe vector, IncP replicon, lacZ Tcr Tra− | 21 |
| pMP220::PpvdA | pvdA promoter directionally cloned in pMP220 (formerly designated pPV51) | 9 |
| pMP220::PpsbA | psbA promoter from pMP190::PpsbA directionally cloned in pMP220 | This study |
| pMP190 | Broad-host-range, low-copy-number promoter-probe vector, IncQ replicon, lacZ Cmr Tra− | 21 |
| pMP190::PpsbA | psbA promoter directionally cloned in pMP190 | 8 |
| pMP190::PpsbS | 470-bp PCR-generated fragment encompassing the psbS promoter directionally ligated to the KpnI-SalI sites of pMP190 | 8 |
| pMP220::PpsbS | DNA fragment of ca. 530 bp excised from pMP190::PpsbS by HindIII digestion and directionally ligated to the HindIII site of pMP220 | This study |
| pMP220::PpvdS | 587-bp PCR-generated fragment encompassing the pvdS promoter directionally ligated to the BglII-EcoRI sites of pMP220 | This study |
| pUCP18 | Escherichia coli-Pseudomonas shuttle vector derived from pUC18, pMB1, replicon pRO1600 lacZα bla Mob+ Tra− Apr Cbr | 19 |
| pCAΔSh | pUCP18-derivative containing a 2.7-kb PstI-SphI fragment encompassing the entire psbA gene | 2 |