FIG. 2.

Variation of total polymer content (○) and polymer composition (•) in JE4199 harboring pMMB-pha and pBAD-Cro with arabinose induction. Cells were grown in MOPS-buffered minimal medium containing 274 mM glycerol with 0.1% succinate (A) and without succinate (B). Expression of the plasmid-encoded genes was induced with 500 μM IPTG and different arabinose concentrations when the cell density reached an OD₆₀₀ of 0.15. The points obtained when no arabinose was added are plotted at 0.00001% (wt/vol) arabinose because zero arabinose could not be plotted on the logarithmic scale.