FIG. 3.

Variation of total polymer content (○) and polymer composition (•) in JE4199 harboring pMMB-pha and pBAD-Cro with external CN-B₁₂ (precursor of coenzyme B₁₂). Cells were grown in MOPS-buffered minimal medium containing 274 mM glycerol. Expression of the plasmid-encoded genes was induced with 500 μM IPTG and 0.1% arabinose when the cell density reached an OD₆₀₀ of 0.15.