TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara,leu)7697 galU galK λ−rpsL nupG | Gibco |
| Salmonella enterica serovar Typhimurium JR501 | hsdSA29 hsdSB121 hsdL6 trpC2 metA22 metE551 ilv452 leu3121 rpsL120 galE719 xyl-404 H1-b H2-e,n,x nml (Fels2)−fla-66 | 61 |
| LT2 derivatives | ||
| TR6583 | metE205 ara-9 | 62 |
| JE4199 | metE205 ara-9 prpC::MudJ | 62 |
| Plasmids | ||
| pBAD24 | Apr, PBAD, araC, pBR322 ori | 17 |
| pMMB206 | Cmr, PtaclacUV5, lacIq, RSF1010 ori | 39 |
| pTrc99A | Apr, Ptrc, lacIq, pBR322 ori | Amersham Pharmacia |
| pBAD-Ae-pha | Apr, R. eutropha PHA synthesis operon (phaCAB) in pBAD24 | 1 |
| pISA1 | Apr, Acinetobacter sp. strain RA3849 PHA operon (phaBCA) in pBAD24 | 1 |
| pMMB-pha | Cmr, Acinetobacter PHA synthesis operon from pISA1 in pMMB206 | This study |
| pTrc-Cro | Apr, operon including sbm, ygfD, and ygfG in pTrc99A | This study |
| pBAD-Cro | Apr, operon including sbm, ygfD, and ygfG in pBAD24 | This study |