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. 2002 Aug;68(8):3708–3715. doi: 10.1128/AEM.68.8.3708-3715.2002

TABLE 4.

Apparent kinetic constants for dTDP-l-rhamnose biosynthetic enzymes

Enzyme	Substrate	K_m (mM)	k_cat (s⁻¹)
LT2 RmlA^a	dTTP	0.020
	α-d-Glucose 1-phosphate	0.11	30
	dTDP-d-glucose	0.083
	Pyrophosphate	0.15	180
DSM 10155 RmlA	dTTP	0.031 ± 0.0040	8.9 ± 0.24
DSM 10155 RmlA	α-d-Glucose 1-phosphate	0.20 ± 0.036	13 ± 1.1
	dTDP-d-glucose	0.13 ± 0.036	15 ± 2.0
	Pyrophosphate	0.055 ± 0.0082	10 ± 0.32
LT2 RmlB^b	dTDP-d-glucose	0.0072	3.0
DSM 10155 RmlB	dTDP-d-glucose	0.012 ± 0.0016	1.9 ± 0.079
LT2 RmlC^c	dTDP-6-deoxy-d-xylo-4- hexulose	0.71 ± 0.17	39 ± 6.6
DSM 10155 RmlC	dTDP-6-deoxy-d-xylo-4- hexulose	0.062 ± 0.0063	2.0 ± 0.67
LT2 RmlD^c	NADH	0.0063 ± 0.0019	39 ± 6.6
	NADPH	0.013 ± 0.0020	25 ± 1.1
DSM 10155 RmlD	NADH	0.012 ± 0.0017	1.5 ± 6.6
DSM 10155 RmlD	NADPH	0.0076 ± 0.0015	2.3 ± 0.14

^a

Data from reference 27.

^b

Data from reference 49.

^c

Data from reference 10.