FIG. 1.
Western blot for ClpP levels in wild-type (MC4100) E. coli grown in the presence of SDS. Cells were grown at 37°C in LB with 0, 0.5, 1.0, 2.0, and 4.0% SDS. Cellular proteins were detected with anti-ClpP antibody (17). Lane 1, prestained molecular weight markers. The ClpP band intensities (pixels/mm2) from left to right were as follows: in lane 2, 20,450 (0% SDS); in lane 3, 14,660 (0.5% SDS); in lane 4, 58,190 (1% SDS); in lane 5, 144,550 (2% SDS); and in lane 6, 92,030 (4% SDS). The latter four values correspond to ClpP increases of 0.7-, 3-, 7-, and 5-fold, respectively.