FIG. 3.

Deletion mutant analysis of the mun locus. Deletion mutants of the mun locus constructed in the vector plasmid, pRH100, by PCR are depicted on the left. Filled and open bars represent putative promoters. Two arrowheads in opposite directions indicate terminators. E. faecium IFO13712 transformants harboring the indicated deletion mutant plasmids were tested for production of and immunity against mundticin KS, as described in Materials and Methods. Symbols: +, production of or resistance to mundticin KS; −, no production of or sensitivity to mundticin KS. ND, not determined.