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Western blot using anti-CtermLIP serum of total cell extract (C) (0.1 U) and supernatant (S) (0.5 U) of the control strain, MG-Pnis-Lip+, the strain overproducing PmpA with the lipase, MG-Pnis::pmpA-Lip+, and the pmpA mutant, MG-pmpA. The addition of the nisin inducer in the growing medium is indicated by -nis or +nis. See the legend to Fig. 3 for an explanation of U.
