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In situ identification of the chlamydia-like bacteria UWE25 within free-living amoebae by FISH using Cy3-labeled probe Bn9658 specific for a subgroup of the Parachlamydiaceae (A) (red) and Cy5-labeled probe Chls-523, which targets all chlamydiae (B) (blue). The FLUOS-labeled probe EUK516, which hybridizes to most eukaryotes (C) (green), was used to stain the amoebal host cells. (D) The chlamydiae appear purple in the overlap image. Bar, 10 μm.
