TABLE 1.
Oligonucleotide probe sequences specific for the Chlamydiales and formamide concentration in the hybridization buffer required for specific FISH
| Probea | Target organisms | Sequence (5′-3′) | % FA | Reference |
|---|---|---|---|---|
| S-O-Chls-0523-a-A-18b | Chlamydiales | CCTCCGTATTACCGCAGC | 25 | This study |
| S-F-Chlae-0574-a-A-18 | Chlamydiaceae | CTTTCCGCCTACACGCCC | 20 | This study |
| S-G-Chla-0232-A-18 | Chlamydia | TAGCTGATATCACATAGA | 10 | This study |
| S-G-Chlph-0583-a-A-18 | Chlamydophila | CTAACTTTCCTTTCCGCC | 25 | This study |
| S-S-Ct-0623-a-A-18 | C. trachomatis | ATTAGATGCCGACTCGGG | 30 | This study |
| S-S-Cpn-0214-a-A-18c | C. pneumoniae | CTCTTCCTCAACCGAAAG | 30 | This study |
| S-S-Cpn-0974-a-A-18d | C. pneumoniae | AAGTCCAGGTAAGGTCCT | 30 | This study |
| S-S-Cps-1414-a-A-18 | “C. psittaci” groupe | AAGGCAAAACCAACTCCC | 30 | This study |
| S-S-Cps-1353-a-A-18 | “C. psittaci” groupe | GGCGTTATAGCTGACACG | 30 | This study |
| Bn9658 | Subgroup of the Parachlamydiaceae | TCCGTTTTCTCCGCCTAC | 10f | Amann et al. (2) |
| S-*-ParaC-0658-a-A-18 | Subgroup of the Parachlamydiaceae | TCCATTTTCTCCGTCTAC | 10f | Horn et al. (22) |
a
Probe designation according to Alm et al. (1); boldface type indicates the probe name as used in the text.
b
To be used in combination with competitor (5′-CCTCCGTATTACCGCGGC-3′).
c
To be used in combination with competitors (5′-CTCTCCCTCAACCGAAAG-3′ and 5′-CTCTTCCCCAACCGAAAG-3′).
d
To be used in combination with competitor (5′-AAACCCAGGTAAGGTCCT-3′).
e
C. psittaci, including C. felis, C. abortus, and C. caviae.
f
Formamide concentration when probes Bn9658 and S-*-ParaC-0658-a-A-18 are used simultaneously.