TABLE 1.
Effect of lactate on the viability of various O157 and non-O157 E. coli strains
| Strain | Origin | Serotype | Decrease in viabilitya
|
|
|---|---|---|---|---|
| d-Lactate | l-Lactate | |||
| NCTC 12900 | Human | O157:H7 (VT−)b | −0.08 | 10.2 |
| NCTC 13126 | Human | O157:H7 (VT−) | 0.92 | 8.52 |
| NCTC 12079 | Human | O157:H7 | 7.6 | 6.40 |
| AUIO-5c | Cattle feces | O157:H7 | 0.42 | 4.66 |
| AUIO-7c | Raw milk | O157:H7 | 0.08 | 4.77 |
| AUIO-13c | Minced beef | O157:H7 | 3.53 | 6.00 |
| AUIO-309c | Cheese | O157:H7 | 0.17 | 4.76 |
| AUIO-NDc | Sheep feces | O157:H7 | 1.42 | 4.49 |
| F318 | Sheep rumen | O162 | 1.25 | 10.4 |
| F38 | Sheep rumen | O rough | 4.08 | 8.83 |
| EC17 | Pig | O106:NM | −0.25 | 10.6 |
| EC30 | Bison | O113:H21 | 6.83 | 16.1 |
| EC33 | Sheep | O7:H21 | 2.00 | 15.4 |
| EC45 | Pig | ON:HM | 1.58 | 13.2 |
| EC47 | Sheep | ON:H18 | −2.08 | 5.75 |
| EC67 | Goat | O4:H43 | 0.08 | 9.42 |
a
Decrease in viable cell numbers, expressed in CFU (108) ml−1, following 3 h of incubation in 100 mM lactate at pH 3.8. Negative values indicate growth. Results are the average of two repeat experiments, and the average standard deviation was 1.54 × 108 CFU ml−1.
b
VT−, verocytotoxin negative.
c
Kindly supplied by Ian Ogden, Department of Medical Microbiology, University of Aberdeen, Aberdeen Royal Hospitals Trust, Foresterhill, Aberdeen, United Kingdom.