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Strategy used for the isolation of the gtfA gene and surrounding regions from L. reuteri strain 121 chromosomal DNA. Primers are indicated with small, black arrows. (A) The fragment given is the 659-bp insert isolated with degenerated primers. (B and D) The fragments are regions amplified by inverse PCR (5,229 and 4,229 bp, respectively). (C) The fragment (2,186 bp) was obtained by colony blot hybridization of a partial gene library.
