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. Author manuscript; available in PMC: 2025 Sep 4.
Published in final edited form as: Cell. 2025 Apr 4;188(11):2957–2973.e28. doi: 10.1016/j.cell.2025.03.029

Key resources table

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Anti-PCDH10 antibody Proteintech Cat#: 21859-1-AP; RRID: AB_2878929
Anti-VLDLR antibody GeneTex Cat#: GTX79552; RRID: AB_11171827
Anti-MXRA8 antibody MBL International Cat#: W040-3; RRID: AB_2801291
Rabbit IgG isotype Proteintech Cat#: 30000-0-AP; RRID: AB_2819035
Mouse IgG isotype BD Biosciences Cat#: BDB557351; RRID: AB_10050406
PE-conjugated donkey anti-rabbit F(ab’)2 fragment Jackson ImmunoResearch Cat#: 711-116-152; RRID: AB_2340599
PE-conjugated donkey anti-mouse F(ab’)2 fragment Jackson ImmunoResearch Cat#: 715-116-150; RRID: AB_2340798
APC-conjugated rat anti-DYKDDDDK (Flag) antibody BioLegend Cat#: 637307; RRID: AB_2561496
APC-conjugated rat isotype control antibody BioLegend Cat#: 402306; RRID: AB_3097097
Anti-Human IgG (Fc specific)-Peroxidase antibody Sigma Cat#: A0170; RRID: AB_257868
Anti-WEEV E2-E1 monoclonal antibody SKW11 Sutton et al.59 N/A
Bacterial and virus strains
Highlands J virus (strain B 230) ATCC Cat#: VR-622
Chemicals, peptides, and recombinant proteins
DMEM Gibco Cat#: C11995500BT
Expi293™ Expression Medium Thermo Fisher Scientific Cat#: A1435101
Neurobasal medium Thermo Fisher Scientific Cat#: 21103
Glutamax Thermo Fisher Scientific Cat#: 35050
B-27 Thermo Fisher Scientific Cat#: 17504
Y-27632 Stemcell Technologies Cat#: 72302
Fetal bovine serum (FBS) ATLAS Biologicals Cat#: F-0500-D
HEPES Thermo Fisher Scientific Cat#: 15630080
RPMI1640 Thermo Fisher Scientific Cat#: 11835055
Pen strep (penicillin-streptomycin) Gibco Cat#: 10378016
Opti-MEM Thermo Fisher Scientific Cat#: 31985088
Dulbecco’s Phosphate Buffered Saline (DPBS) Thermo Fisher Scientific Cat#: 14190-144
Pierce Chromogenic Endotoxin Quantification Kit Thermo Fisher Scientific Cat# A39552
MabSelectTM PrismA protein A affinity resin Cytiva Cat#: 17549801
Superdex 200 increase 10/300 GL column Cytiva Cat#: 28990944
Human PCDH10EC1-Fc Li et al.15 N/A
Human VLDLRLBD-Fc Clark et al.34 N/A
Human VLDLR LA(1-2)-Fc This study N/A
Sparrow PCDH10EC1-Fc This study N/A
Human MXRA8ect-Fc Clark et al.34 N/A
Human PCDH10EC1-twin-strep This study N/A
Receptor associated protein (RAP) Clark et al.34 N/A
Critical commercial assays
ExpiFectamineTM 293 Transfection Kit Thermo Fisher Scientific Cat#: A14525
Lipofectamine 3000 Thermo Fisher Scientific Cat#: L3000150
e-Myco PCR detection kit Bulldog Bio Cat#: 25234
Deposited data
WEEV CBA87 VLP density map This study EMD-47117
WEEV CBA87 VLP model This study PDB ID: 9DQX
WEEV CBA87 VLP/human PCDH10EC1-Fc density map This study EMD-47116
WEEV CBA87 VLP/human PCDH10EC1-Fc model This study PDB ID: 9DQV
WEEV Imperial 181 VLP/sparrow PCDH10EC1-Fc density map This study EMD-47118
WEEV Imperial 181 VLP/sparrow PCDH10EC1-Fc model This study PDB ID: 9DQY
WEEV McMillan VLP/VLDLRLBD-Fc density map This study EMD-47119
WEEV McMillan VLP/ VLDLRLBD-Fc model This study PDB ID: 9DQZ
Experimental models: Cell lines
Expi293F™ cells Thermo Fisher Scientific Cat#: A14527, RRID: CVCL_D615
HEK 293T (human kidney epithelial) ATCC Cat#: CRL-11268, RRID:CVCL_0063
Vero E6 (Cercopithecus aethiops kidney epithelial) ATCC Cat#: CRL-1586, RRID: CVCL_0574
K562 (human chronic myelogenous leukemia) ATCC Cat#: CCL-243, RRID:CVCL_0004
Embryonic day 17 mouse cortical neurons Thermo Fisher Scientific Cat#: A15586
K562 cells expressing P. domesticus PCDH10 Li et al.15 N/A
K562 cells expressing E. caballus PCDH10 Li et al.15 N/A
K562 cells expressing P. domesticus MXRA8 This study N/A
K562 cells expressing M. musculus PCDH10 Li et al.15 N/A
K562 cells expressing T. sirtalis PCDH10 Li et al.15 N/A
Experimental models: Organisms/strains
CD-1 IGS mice Charles River Strain Code 022
Recombinant DNA
pVRC-human PCDH10EC1-Fc Li et al.15 N/A
pVRC-sparrow PCDH10EC1-Fc This study N/A
pVRC-human VLDLRLBD-Fc Clark et al.34 N/A
pVRC-human MXRA8ect-Fc Clark et al.34 N/A
pVRC-human PCDH10EC1-twin-strep This study N/A
pCAGGS-human RAP Clark et al.34 N/A
pCAGGS-WEEV 71V1658 E3-E2-6K/TF-E1 (and mutants) Li et al.15 and this study N/A
pCAGGS-WEEV Fleming E3-E2-6K/TF-E1 (and mutants) Li et al.15 and this study N/A
pCAGGS-WEEV McMillan E3-E2-6K/TF-E1 (and mutants) Li et al.15 and this study N/A
pCAGGS-WEEV Imperial 181 E3-E2-6K/TF-E1 (and mutants) Li et al.15 and this study N/A
pCAGGS-WEEV CBA87 E3-E2-6K/TF-E1 Li et al.15 N/A
pCAGGS-WEEV BFS09997 E3-E2-6K/TF-E1 This study N/A
pCAGGS-WEEV EP6 E3-E2-6K/TF-E1 This study N/A
pCAGGS-WEEV AG80-646 E3-E2-6K/TF-E1 This study N/A
pCAGGS-WEEV TR25717 E3-E2-6K/TF-E1 This study N/A
pCAGGS-WEEV EQ1090 E3-E2-6K/TF-E1 This study N/A
pCAGGS-WEEV DILAVE218 E3-E2-6K/TF-E1 This study N/A
pCAGGS-HJV 585-01 E3-E2-6K/TF-E1 (and mutants) This study N/A
lentiGuide-Puro-human PCDH10 Li et al.15 N/A
lentiGuide-Puro-human PCDH10 stalk-Flag Li et al.15 N/A
lentiGuide-Puro-human PCDH10 EC1-Flag (and mutants) Li et al.15 N/A
lentiGuide-Puro-human VLDLR Clark et al.34 N/A
lentiGuide-Puro-human VLDLR ΔLBD-Flag Clark et al.34 N/A
lentiGuide-Puro-human VLDLR Flag-tagged single LA repeat Yang et al.24 N/A
lentiGuide-Puro-human MXRA8 Clark et al.34 N/A
lentiGuide-Puro-human ApoER2 iso2 Clark et al.34 N/A
lentiGuide-Puro-sparrow PCDH10 Li et al.15 N/A
lentiGuide-Puro-sparrow MXRA8 This study N/A
lentiGuide-Puro-garter snake PCDH10 Li et al.15 N/A
lentiGuide-Puro-mouse PCDH10 Li et al.15 N/A
lentiGuide-Puro horse PCDH10 Li et al.15 N/A
WEEV CBA87 virus-like particle in pVRC plasmid Ko et al.32 N/A
WEEV McMillan virus-like particle in pVRC plasmid Li et al.15 N/A
WEEV Imperial 181 virus-like particle in pVRC plasmid This study N/A
Western equine encephalitis virus (strain McMillan) molecular clone Gardner et al.60
Software and algorithms
RELION v3.1.4 Zivanov et al.61 N/A
crYOLO v1.8.2 Wagner et al.62 N/A
DeepEMhancer v20210511 Sanchez-Garcia et al.63 N/A
MotionCor2 v1.6.4 Zheng et al.64 N/A
CTFFIND-4.1 v4.1.14 Rohou et al.65 N/A
UCSF ChimeraX v1.6.1 Pettersen et al.66 N/A
PyMOL v3.0.2 (https://www.pymol.org/pymol) N/A
Coot v0.9.8 Emsley et al.67 N/A
Phenix v1.21rc1-5127 Adams et al.68 N/A
AlphaFold2 Jumper et al.69 N/A
GraphPad Prism v10.2.3 GraphPad N/A
FlowJo v10.6.2 FlowJo, LLC N/A
Incucyte S3 Software v2023B Sartorius N/A
MEGA v11.0.10 MEGA Development Team N/A
Other
Quantifoil grids R 0.6/1 300 mesh, gold Electron Microscopy Sciences Cat#: Q350AR-06
Quantifoil grids R 2/2 300 mesh, gold Electron Microscopy Sciences Cat#: Q3100AR2