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. 2002 Sep;68(9):4583–4592. doi: 10.1128/AEM.68.9.4583-4592.2002

FIG. 3.

FIG. 3.

Expression of S. litura APN in Sf21 cells. (A) One million uninfected and 72-h-infected cells were harvested, and total cell extract was prepared by resuspending and boiling the cells in SDS sample buffer containing β-mercaptoethanol. (B) Membranes were isolated from both uninfected and infected cells by the procedure described in Materials and Methods. Cell extracts and equal amounts of membrane proteins were resolved by SDS-7.5% PAGE and stained with Coomassie brilliant blue. Lanes 1 and 3, uninfected Sf21 cells; lanes 2 and 4, BV-SlApn-infected cells. The positions of molecular mass markers (in kilodaltons) are indicated on the left.