FIG. 8.

Identification of Cry1C toxin-binding protein in S. litura BBMV. (A) Twenty-five micrograms of BBMV proteins was resolved by SDS-PAGE and transferred to nitrocellulose membrane. The membrane strips were incubated with anti-Cry1C antiserum without prior overlaying with Cry1C toxin (lane 1), with Cry1C toxin and anti-Cry1C antiserum (lane 2), and with anti-APN antibodies (lane 3). (B) Sf21-expressed S. litura APN was transferred to the membrane and probed with anti-APN antibodies. The membranes were then incubated with alkaline phosphatase-conjugated secondary antibody and NBT-BCIP substrate as described in Materials and Methods. The positions of molecular mass markers (in kilodaltons) are indicated on the left.