FIG. 9.

Ligand blot analysis of insect cells expressing S. litura APN. Membrane proteins (2.5 μg) prepared from uninfected Sf21 cells (lane 1) and BV-SlApn-infected cells (lane 2 and 3) were solubilized in SDS sample buffer containing β-mercaptoethanol, separated by SDS-7.5% PAGE, and electrotransferred to nitrocellulose membrane. The nitrocellulose membrane was probed with Cry1C (1 μg/ml) (lanes 1 and 2) and Cry1Ac (1 μg/ml) (lane 3) δ-endotoxins. Binding of the toxin was detected by using antibodies against the toxin followed by alkaline phosphatase-conjugated goat anti-rabbit IgG. The positions of molecular mass markers (in kilodaltons) are indicated on the left.