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Action of GDE on amylose with average molecular mass of 30 kDa. A reaction mixture (2 ml) containing 2 mg of synthetic amylose AS-30, 20 mM sodium acetate buffer (pH 6.0), and 1.0 U of GDE was incubated at 40°C. At the indicated time points, 300 μl of the mixture was removed, boiled for 5 min to stop the reaction, centrifuged to remove insoluble materials, and then subjected to analyses.
