FIG. 3.

HPAEC analysis of GA-resistant molecules. A 100-μl reaction mixture described in Fig. 2 was treated with a mixture of GA, isoamylase, and pullulanase. The GA-resistant molecules were precipitated by adding 10 volumes of acetone. Cycloamylose with a DP of 6 can be quantitatively precipitated by this treatment. (A) The pellet was dried, redissolved in 7.5 μl of 1 M NaOH, diluted to 50 μl with distilled water, and then subjected to HPAEC analysis. (B) Cycloamylose with DP between 6 and 31 (0.5 nmol each) are shown as standards.