FIG. 7.

HPAEC analysis of cycloamylose produced by GDE (A), D-enzyme plus isoamylase (B), or D-enzyme (C) from soluble starch. A reaction mixture (2.5 ml) containing 0.1% (wt/vol) soluble starch, 0.625 U of GDE, and 10 mM sodium acetate buffer (pH 6.0) was incubated at 40°C. As controls, 875 U of D-enzyme or enzyme mixture (875 U of D-enzyme, 4 U of isoamylase) was used in place of GDE. The cycloamyloses were prepared from 24-h reaction mixture as described in the legend to Fig. 2.