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. 2002 Mar;110(3):229–233. doi: 10.1289/ehp.02110229

Environmental organochlorines and semen quality: results of a pilot study.

Russ Hauser 1, Larisa Altshul 1, Zuying Chen 1, Louise Ryan 1, James Overstreet 1, Isaac Schiff 1, David C Christiani 1
PMCID: PMC1240761  PMID: 11882472

Abstract

There have been numerous studies that suggest that sperm concentrations (sperm counts) are declining in men. However, other studies suggest that sperm counts are not declining or may be increasing in some areas. Although there is disagreement on whether there is a downward temporal trend in sperm counts, the studies provide evidence that sperm counts vary by geographic location. It has been hypothesized that the geographic variation in sperm concentrations may be due to environmental exposures, lifestyle factors, or some unknown causes. To determine whether contemporary ambient levels of polychlorinated biphenyls (PCBs) and p,p-DDE are associated with altered semen quantity and quality, we selected a study population without specific exposure to PCBs or p,p-DDE. The present study presents the results from a pilot study on the relationship between serum PCBs and p,p-DDE and semen quality in 29 subjects recruited from the Massachusetts General Hospital Andrology Laboratory. Of the 29 subjects, 3 had sperm concentrations < 20 million/mL, 7 had < 50% motile sperm, 9 had < 4% normal morphology, and 6 were below normal in more than one semen parameter. The 18 subjects with normal spermatozoa concentration, motility, and morphology were used as comparison subjects. The mean (SE) concentration of the sum of PCBs and p,p-DDE was 242 ng/g lipids (34.0) and 354 ng/g lipids (120), respectively, for men with below normal motility as compared to 202 ng/g lipids (16.6) and 240 ng/g lipids (31.1), respectively, for the comparison subjects. The data showed general trends that were suggestive of an association between PCBs and p,p-DDE and abnormal motility, as well as with sperm concentration and morphology. A full-scale study is currently in progress.

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Selected References

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  1. Auger J., Kunstmann J. M., Czyglik F., Jouannet P. Decline in semen quality among fertile men in Paris during the past 20 years. N Engl J Med. 1995 Feb 2;332(5):281–285. doi: 10.1056/NEJM199502023320501. [DOI] [PubMed] [Google Scholar]
  2. Bujan L., Mansat A., Pontonnier F., Mieusset R. Time series analysis of sperm concentration in fertile men in Toulouse, France between 1977 and 1992. BMJ. 1996 Feb 24;312(7029):471–472. doi: 10.1136/bmj.312.7029.471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bush B., Bennett A. H., Snow J. T. Polychlorobiphenyl congeners, p,p'-DDE, and sperm function in humans. Arch Environ Contam Toxicol. 1986 Jul;15(4):333–341. doi: 10.1007/BF01066399. [DOI] [PubMed] [Google Scholar]
  4. Bush B., Snow J., Koblintz R. Polychlorobiphenyl (PCB) congeners, p,p'-DDE, and hexachlorobenzene in maternal and fetal cord blood from mothers in Upstate New York. Arch Environ Contam Toxicol. 1984 Sep;13(5):517–527. doi: 10.1007/BF01056331. [DOI] [PubMed] [Google Scholar]
  5. Carlsen E., Giwercman A., Keiding N., Skakkebaek N. E. Evidence for decreasing quality of semen during past 50 years. BMJ. 1992 Sep 12;305(6854):609–613. doi: 10.1136/bmj.305.6854.609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davis R. O., Katz D. F. Operational standards for CASA instruments. J Androl. 1993 Sep-Oct;14(5):385–394. [PubMed] [Google Scholar]
  7. DeVoto E., Fiore B. J., Millikan R., Anderson H. A., Sheldon L., Sonzogni W. C., Longnecker M. P. Correlations among human blood levels of specific PCB congeners and implications for epidemiologic studies. Am J Ind Med. 1997 Dec;32(6):606–613. doi: 10.1002/(sici)1097-0274(199712)32:6<606::aid-ajim6>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
  8. Endtz A. W. A rapid staining method for differentiating granulocytes from "germinal cells" in Papanicolaou-stained semen. Acta Cytol. 1974 Jan-Feb;18(1):2–7. [PubMed] [Google Scholar]
  9. Fisch H., Goluboff E. T. Geographic variations in sperm counts: a potential cause of bias in studies of semen quality. Fertil Steril. 1996 May;65(5):1044–1046. doi: 10.1016/s0015-0282(16)58284-3. [DOI] [PubMed] [Google Scholar]
  10. Fisch H., Goluboff E. T., Olson J. H., Feldshuh J., Broder S. J., Barad D. H. Semen analyses in 1,283 men from the United States over a 25-year period: no decline in quality. Fertil Steril. 1996 May;65(5):1009–1014. doi: 10.1016/s0015-0282(16)58278-8. [DOI] [PubMed] [Google Scholar]
  11. Giwercman A., Carlsen E., Keiding N., Skakkebaek N. E. Evidence for increasing incidence of abnormalities of the human testis: a review. Environ Health Perspect. 1993 Jul;101 (Suppl 2):65–71. doi: 10.1289/ehp.93101s265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Irvine S., Cawood E., Richardson D., MacDonald E., Aitken J. Evidence of deteriorating semen quality in the United Kingdom: birth cohort study in 577 men in Scotland over 11 years. BMJ. 1996 Feb 24;312(7029):467–471. doi: 10.1136/bmj.312.7029.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jansen H. T., Cooke P. S., Porcelli J., Liu T. C., Hansen L. G. Estrogenic and antiestrogenic actions of PCBs in the female rat: in vitro and in vivo studies. Reprod Toxicol. 1993 May-Jun;7(3):237–248. doi: 10.1016/0890-6238(93)90230-5. [DOI] [PubMed] [Google Scholar]
  14. Kelce W. R., Stone C. R., Laws S. C., Gray L. E., Kemppainen J. A., Wilson E. M. Persistent DDT metabolite p,p'-DDE is a potent androgen receptor antagonist. Nature. 1995 Jun 15;375(6532):581–585. doi: 10.1038/375581a0. [DOI] [PubMed] [Google Scholar]
  15. Korach K. S., Sarver P., Chae K., McLachlan J. A., McKinney J. D. Estrogen receptor-binding activity of polychlorinated hydroxybiphenyls: conformationally restricted structural probes. Mol Pharmacol. 1988 Jan;33(1):120–126. [PubMed] [Google Scholar]
  16. Korrick S. A., Altshul L. M., Tolbert P. E., Burse V. W., Needham L. L., Monson R. R. Measurement of PCBs, DDE, and hexachlorobenzene in cord blood from infants born in towns adjacent to a PCB-contaminated waste site. J Expo Anal Environ Epidemiol. 2000 Nov-Dec;10(6 Pt 2):743–754. doi: 10.1038/sj.jea.7500120. [DOI] [PubMed] [Google Scholar]
  17. Kruger T. F., Acosta A. A., Simmons K. F., Swanson R. J., Matta J. F., Oehninger S. Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril. 1988 Jan;49(1):112–117. doi: 10.1016/s0015-0282(16)59660-5. [DOI] [PubMed] [Google Scholar]
  18. Longnecker M. P., Rogan W. J., Lucier G. The human health effects of DDT (dichlorodiphenyltrichloroethane) and PCBS (polychlorinated biphenyls) and an overview of organochlorines in public health. Annu Rev Public Health. 1997;18:211–244. doi: 10.1146/annurev.publhealth.18.1.211. [DOI] [PubMed] [Google Scholar]
  19. Murphy R., Harvey C. Residues and metabolites of selected persistent halogenated hydrocarbons in blood specimens from a general population survey. Environ Health Perspect. 1985 May;60:115–120. doi: 10.1289/ehp.8560115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Paulsen C. A., Berman N. G., Wang C. Data from men in greater Seattle area reveals no downward trend in semen quality: further evidence that deterioration of semen quality is not geographically uniform. Fertil Steril. 1996 May;65(5):1015–1020. [PubMed] [Google Scholar]
  21. Raychoudhury S. S., Flowers A. F., Millette C. F., Finlay M. F. Toxic effects of polychlorinated biphenyls on cultured rat Sertoli cells. J Androl. 2000 Nov-Dec;21(6):964–973. [PubMed] [Google Scholar]
  22. Sharpe R. M., Skakkebaek N. E. Are oestrogens involved in falling sperm counts and disorders of the male reproductive tract? Lancet. 1993 May 29;341(8857):1392–1395. doi: 10.1016/0140-6736(93)90953-e. [DOI] [PubMed] [Google Scholar]
  23. Sherins R. J. Are semen quality and male fertility changing? N Engl J Med. 1995 Feb 2;332(5):327–328. doi: 10.1056/NEJM199502023320510. [DOI] [PubMed] [Google Scholar]
  24. Stehr-Green P. A. Demographic and seasonal influences on human serum pesticide residue levels. J Toxicol Environ Health. 1989;27(4):405–421. doi: 10.1080/15287398909531312. [DOI] [PubMed] [Google Scholar]
  25. Sun J. G., Jurisicova A., Casper R. F. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol Reprod. 1997 Mar;56(3):602–607. doi: 10.1095/biolreprod56.3.602. [DOI] [PubMed] [Google Scholar]
  26. Swan S. H., Elkin E. P., Fenster L. Have sperm densities declined? A reanalysis of global trend data. Environ Health Perspect. 1997 Nov;105(11):1228–1232. doi: 10.1289/ehp.971051228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Swan S. H., Elkin E. P., Fenster L. The question of declining sperm density revisited: an analysis of 101 studies published 1934-1996. Environ Health Perspect. 2000 Oct;108(10):961–966. doi: 10.1289/ehp.00108961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Vine M. F., Margolin B. H., Morrison H. I., Hulka B. S. Cigarette smoking and sperm density: a meta-analysis. Fertil Steril. 1994 Jan;61(1):35–43. [PubMed] [Google Scholar]

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