Fig. 2:

B. subtilis Rho terminates pyrimidine-rich antisense sequence. a, As in Fig. 1d, but for fragments that fully overlap with the sense (blue) or antisense (green) strand of annotated genes. Legend indicates total number of each fragment type. Top left, count of each fragment type in Rho-terminated fragments. Contour lines mark 10^th, 25^th, 50^th, 75^th percentiles of Gaussian kernel density estimates, excluding points below detection. b, Sequence features of 1,500 non-terminated fragments (>500 reads pre-selection) and 1,500 Rho-terminated fragments colored by enrichment in the absence of BCM (fragment length ≥ 150 bp). Excess C quantifies C content relative to G content. Maximum %T is maximum T content in 150 nt windows tiling the sequence (Methods). Dashed line indicates the predicted boundary for Rho-terminated sequences (Rho target score = 10⁰) (Methods). c, Beta-galactosidase (LacZ) assays for termination of recoded brnQ variants in wild-type and Δrho backgrounds. Top right, reporter schematic. Ins., insert; lacZ, beta-galactosidase gene. Left, image of B. subtilis reporter strains with indicated insert spotted on an X-gal plate (Methods). Percent of nucleotides substituted indicated in parentheses (insert sequences will be available in Supplementary Table 2). Heatmaps below each brnQ sequence show the Rho target score in 300-nt sliding windows (Methods). Colorbar range: 0.2 to 5 over log scale. Colored lines summarize result: grey, no termination; purple, Rho termination; pink, Rho-independent termination. Uncropped plate image in Extended Data Fig. 4a. d, Distribution of sequence features of the sense fragments shown in a, excluding fragments shorter than 150 nt (n = 46,777 fragments) (Methods). Dashed line as in b.