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Growth of S. cerevisiae (a) and extracellular glycerol production (b) during shake flask cultivation in glucose-YNB medium at 30°C. Each data point shows the mean of triplicate determinations, with error bars indicating the standard error. Values for the growth curve and change in extracellular glycerol concentration were fitted to a five-parameter sigmoidal function (correlation coefficients [r2] were above 0.989 for both curves).
