TABLE 2.

Fixed metabolite concentrations of the kinetic model

Metabolite	Intracellular concn (mM) at the following growth phasea:			Published intracellular concn value (mM)	Reference(s)
	Early exponentialb	Mid-exponentialc	Early stationaryd
ATP	2.37 ± 0.12	2.25 ± 0.26	0.4 ± 0.03	0.04-4.8	18, 52
ADP	2.17 ± 0.20	1.31 ± 0.21	0.76 ± 0.07	0.4-4.2	23, 49
NADH	1.87 ± 0.24	0.62 ± 0.40	0.33 ± 0.02	0.23-1.2	23, 49
NAD+	1.45 ± 0.17	0.47 ± 0.09	0.48 ± 0.04	0.8-5.0	23, 49
F1,6BP	6.01 ± 0.35	3.12 ± 0.58	1.27 ± 0.06	0.5	18
DHAP	0.59 ± 0.07	0.31 ± 0.09	0.14 ± 0.02	0.4	18
Glycerol	15.10 ± 1.06	21.00 ± 0.99	6.72 ± 0.26	24	54

^aValues were derived from experiments performed in triplicate and are presented as the average with standard deviation.

^bOD₆₀₀, 0.4 [400 to 430 min].

^cOD₆₀₀, 0.7 [600 to 630 min].

^dOD₆₀₀, 1.1 [970 to 1,000 min].