TABLE 3.
DAPI and PI total cell counts, Erec482 probe hybridization counts, and Urobe63 probe hybridization counts for fecal samples from three individuals taken over a 4-week period, as determined by microscopy and flow cytometry
Individual | Time (weeks) | DAPI or PI counts
|
Erec482 counts
|
Urobe63 counts
|
||||||
---|---|---|---|---|---|---|---|---|---|---|
Microscopy (1011)a | Flow cytometry (1011)a | Ratiob | Microscopy (1010)a | Flow cytometry (1010)a | Ratiob | Microscopy (1010)a | Flow cytometry (1010)a | Ratiob | ||
A | 0 | 1.87 ± 0.17 | 2.40 ± 0.54 | 0.78 | 3.95 ± 0.63 | 2.80 ± 1.16 | 1.41 | 1.06 ± 0.17 | 0.55 ± 0.36 | 1.93 |
2 | 3.27 ± 0.40 | 4.98 ± 0.52 | 0.66 | 4.32 ± 0.77 | 5.69 ± 0.73 | 0.76 | 1.06 ± 0.30 | 1.33 ± 0.31 | 0.79 | |
4 | 4.06 ± 0.47 | 5.20 ± 2.60 | 0.78 | 4.90 ± 1.22 | 5.16 ± 0.75 | 0.95 | 1.06 ± 0.25 | 0.72 ± 0.12 | 1.47 | |
B | 0 | 3.55 ± 0.41 | 4.72 ± 0.71 | 0.75 | 8.35 ± 1.00 | 7.16 ± 1.76 | 1.17 | 0.56 ± 0.07 | 0.88 ± 0.30 | 0.64 |
2 | 3.42 ± 0.37 | 3.30 ± 0.59 | 1.04 | 5.93 ± 0.69 | 5.15 ± 1.45 | 1.15 | 0.88 ± 0.20 | 1.31 ± 0.37 | 0.67 | |
4 | 3.24 ± 0.33 | 3.15 ± 0.95 | 1.03 | 8.30 ± 1.90 | 4.98 ± 1.51 | 1.67 | 0.71 ± 0.17 | 0.75 ± 0.12 | 0.94 | |
C | 0 | 2.27 ± 0.41 | 2.06 ± 0.64 | 1.10 | 3.55 ± 0.58 | 4.45 ± 1.91 | 0.80 | 0.71 ± 0.25 | 0.57 ± 0.19 | 1.25 |
2 | 2.43 ± 0.25 | 2.41 ± 0.97 | 1.00 | 2.75 ± 0.46 | 2.94 ± 0.91 | 0.94 | 0.38 ± 0.06 | 0.31 ± 0.01 | 1.22 | |
4 | 2.23 ± 0.45 | 2.24 ± 1.50 | 1.00 | 2.49 ± 0.62 | 2.96 ± 1.00 | 0.93 | 0.54 ± 0.15 | 0.41 ± 0.16 | 1.30 |
The values are means ± standard deviations and are expressed in cells per gram (dry weight) of feces.
The ratio was calculated by dividing the mean microscopic count by the mean flow cytometric count.