FIG. 1.
Map of pPProGreen (15.2 kb). The promoter proU was directionally cloned from the proU expression vector pOSEX4 (19) into the broad-host-range pPROBE-KT′ vector (38). The rrnB transcriptional terminators, with one copy (T1) downstream and four copies (T4) upstream of the fusion, surrounded the PproU-gfp fusion. The proU promoter contains an upstream activating region (UAR) and a silencer (S) located in the proV gene. The transcriptional (+1) and translational (+64) start sites are shown. All of the restriction enzyme sites shown, including HindIII (H), SalI (S), BamHI (B), and EcoRI (E), were unique in pPProGreen.