TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and/or characteristicsa | Source or reference |
|---|---|---|
| E. coli strains | ||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
| DH5α | F−endA1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrA96 relA1 φ80dlacZΔM15 λ− | 47 |
| Pseudomonas strains | ||
| DSS73 | Wild type | 50 |
| DSS73-15C2 | KmramsY::Tn5 mutant of DSS73 | This study |
| DSS73-12H8 | KmrgacS::Tn5 mutant of DSS73 | This study |
| DSS73-MM | Kmr spontaneous gacS mutant of DSS73-15C2 | This study |
| Plasmids | ||
| p15C2 | Kmr; contains Tn5 and flanking chromosomal DNA from DSS73-15C2 | This study |
| p12H8 | Kmr; contains Tn5 and flanking chromosomal DNA from DSS73-12H8 | This study |
| pRK415 | Tcr; IncP1 replicon; polylinker of pUC19 | 24 |
| pJEL5771 | Tcr; contains functional gacS gene from P. fluorescens Pf5 | 9 |
| pEMH97 | Tcr; contains functional gacS gene from P. syringae pv. syringae B728a | 22 |
| pRL1063 | Kmr; delivery plasmid for Tn5 and luxAB | 58 |
| pRK2013 | Kmr; mobilizing plasmid | 13 |
a
Kmr and Tcr, resistance to kanamycin and tetracycline, respectively.