Table 1.
Classical phenotypic characterization of the four isolated strains (code numbers 24–6,286-7554 for 1st farm, 24–6,281-7551 and 24–6,281-7552 for 2nd farm, and 24–6,283-7553 for 3rd farm) from the three farms, compared to the reference B. microti CMC4915 strain and B. microti-like 17–2,122-4144 isolated from the marsh frog.
| B. microti CMC4915 | B. microti-like 17–2,122-4144 | 24–6,286-7554 (genital LN sheep 1st farm) | 24–6,283-7553 (retromammary LN sheep 2nd farm) | 24–6,281-7551 (genital LN goat 3rd farm) | 24–6,281-7552 (retromammary LN goat 3rd farm) | |
|---|---|---|---|---|---|---|
| Morphology | S | S | S | S | S | S |
| CO2 | − | − | − | − | − | − |
| H2S | − | − | − | − | − | − |
| Oxidase | + | + | + | + | + | + |
| Urease | + slow | + slow | + slow | + slow | + slow | + slow |
| A | − | + | − | − | − | − |
| M | + | − | + | + | + | + |
| R | − | − | − | − | − | − |
| Thionin | + | + | + | + | + | + |
| Fuchsin | + | + | + | + | + | + |
| Tb RTD | − | − | − | − | − | − |
| Tb 104 RTD | + | + | + | + | + | + |
| Wb RTD | + | + | + | + | + | + |
| Iz RTD | + | + | + | + | + | + |
| R/C RTD | − | − | − | − | − | − |
R/S, Colony morphology (Rough/Smooth), CO2 requirement, H2S production; agglutination with monospecific A, M, and R (rough) antisera; Dye (thionin and basic fuchsin) concentration 20 μg/mL in serum dextrose medium (1/50,000); + = growth or Lysis by phages; − = no growth or lysis.