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. 2025 Aug 21;16:1607131. doi: 10.3389/fmicb.2025.1607131

Figure 9.

Graphs and a heatmap show experimental results. Panel A presents bar graphs of relative mRNA folds for IGF1, PI3K, AKT, P27, and CCND2 across conditions. Panel B shows protein expressions in Western blots for P-PI3K, PI3K, P-AKT, AKT, CCND2, P27KIP1, IGF1, and GAPDH. Panel C features bar graphs for relative protein expression ratios. Panel D is a heatmap depicting data correlations among several bacteria and gene expressions, with red indicating higher correlation and blue lower. Statistical significance is marked with asterisks.

PI3K/Akt signaling pathway expression and correlation analysis. (A) Relative mRNA expression levels of PI3K/Akt signaling pathway-related genes in each group (n = 3). (B) Western blot analysis of hepatic protein levels of p-PI3K, PI3K, p-AKT, AKT, CCND2, P27KIP1, and IGF1. GAPDH was used as the loading control. (C) Quantitative assessment of p-PI3K/PI3K, p-AKT/AKT, CCND2, P27KIP1, and IGF1 protein levels (n = 3). (D) Heatmap depicting Spearman correlations among serum biochemical indices, small intestinal transcriptomics, liver tissue-associated genes, and the gut microbiota. The data are presented as the means ± SEMs. Statistical analysis was performed via ordinary one-way ANOVA. *p < 0.05, **p < 0.005, ***p < 0.001. All experiments were performed in triplicate, with a minimum of three replicates.